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蛋白質(zhì)外聚物中多糖的比例——結(jié)論、致謝!
來源:上海謂載 瀏覽 1546 次 發(fā)布時間:2021-10-12
四、結(jié)論
油和/或 Corexit 的存在會導(dǎo)致 EPS 的蛋白質(zhì):多糖比率更高,并在中胚層實驗中降低 SFT。 在這些實驗中,SFT 與 蛋白質(zhì):具有負(fù)斜率的 EPS 多糖。 當(dāng)開闊的海洋 水域和兩種不同的沿海水處理進行了比較, 蛋白質(zhì)趨勢:多糖為 CEWAF > DCEWAF > WAF ≥ Control 并且對于 SFT,它是相反的, CEWAF < DCEWAF < WAF ≤ 對照。 因此,SFT 與膠體 EPS 中的蛋白質(zhì):多糖比率成反比。
當(dāng)中宇宙水柱的不同尺寸分?jǐn)?shù)為 相比之下,我們發(fā)現(xiàn) EPS 膠體可以降低 SFT 蛋白質(zhì):多糖比例,表明有效的生物乳化 蛋白質(zhì)的容量。 粒子濾波中 SFT 的比較 分?jǐn)?shù) (< 0.45 μm) 和 EPS 膠體分?jǐn)?shù) (< 0.45 μm 和 > 3 kDa),對于真正溶解的部分 (< 3 kDa),它是 表明只有前兩個包含 EPS 的部分具有容量 以降低 SFT,而 < 3 kDa 級分顯示與以下相同的 SFT 純海水或只有真正溶解有機碳的海水。
顯微鏡技術(shù)(即 CLSM 和 SEM)證實,正如預(yù)測的那樣,蛋白質(zhì)主要在空氣 - 水界面富集, 強烈影響空氣/水界面處的 SFT 治療。 這些技術(shù)還可視化了不同的聚集體尺寸 和它們的分散,以及聚集體形成的重要性 通過陰離子EPS組分部分之間的Ca2+"橋接"。 SFT 可能會發(fā)生微小的變化,與蛋白質(zhì):多糖比率的變化相吻合,這可能是 pH 值變化的原因(十分之一) 單位),如 EPS 模型化合物所示,這可能在 CMC 周圍最為突出。 此外,我們表明蛋白質(zhì)和酸性多糖的 EPS 模型成分比 Corexit 導(dǎo)致海水中膠束的自組裝甚至 當(dāng)這些成分的濃度很低時。 這個 表明 EPS 在形成方面與 Corexit 相同或更有效 乳液。 然而,關(guān)于相互作用的更系統(tǒng)的研究 不同組件的不同組合,以及更多型號 單獨的化合物,可能需要更多地闡明在我們的中宇宙實驗中觀察到的復(fù)雜性。
致謝
這項研究得到了墨西哥灣的資助 支持名為 ADDOMEx 的聯(lián)盟研究的研究計劃 (微生物對分散劑和油的聚集和降解 Exopolymers) 聯(lián)盟。 原始數(shù)據(jù)可以在海灣找到 墨西哥研究倡議信息和數(shù)據(jù)合作組織 (GRIIDC) 在網(wǎng)址 https://doi.org/10.7266/N7PK0D64; https://doi.org/10。 7266/N78P5XZD; https://doi.org/10.7266/N74X568X; https://doi. org/10.7266/N79W0D1K。
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